| First Author | Cosette J | Year | 2015 |
| Journal | Sci Rep | Volume | 5 |
| Pages | 17756 | PubMed ID | 26648396 |
| Mgi Jnum | J:337572 | Mgi Id | MGI:6219745 |
| Doi | 10.1038/srep17756 | Citation | Cosette J, et al. (2015) Single Cell Dynamics Causes Pareto-Like Effect in Stimulated T Cell Populations. Sci Rep 5:17756 |
| abstractText | Cell fate choice during the process of differentiation may obey to deterministic or stochastic rules. In order to discriminate between these two strategies we used time-lapse microscopy of individual murine CD4 + T cells that allows investigating the dynamics of proliferation and fate commitment. We observed highly heterogeneous division and death rates between individual clones resulting in a Pareto-like dominance of a few clones at the end of the experiment. Commitment to the Treg fate was monitored using the expression of a GFP reporter gene under the control of the endogenous Foxp3 promoter. All possible combinations of proliferation and differentiation were observed and resulted in exclusively GFP-, GFP+ or mixed phenotype clones of very different population sizes. We simulated the process of proliferation and differentiation using a simple mathematical model of stochastic decision-making based on the experimentally observed parameters. The simulations show that a stochastic scenario is fully compatible with the observed Pareto-like imbalance in the final population. |
