| First Author | Wu T | Year | 2023 |
| Journal | Sci Adv | Volume | 9 |
| Issue | 9 | Pages | eadd2058 |
| PubMed ID | 36857451 | Mgi Jnum | J:334449 |
| Mgi Id | MGI:7441504 | Doi | 10.1126/sciadv.add2058 |
| Citation | Wu T, et al. (2023) A genetically encoded far-red fluorescent indicator for imaging synaptically released Zn(2). Sci Adv 9(9):eadd2058 |
| abstractText | Synaptic zinc ion (Zn(2+)) has emerged as a key neuromodulator in the brain. However, the lack of research tools for directly tracking synaptic Zn(2+) in the brain of awake animals hinders our rigorous understanding of the physiological and pathological roles of synaptic Zn(2+). In this study, we developed a genetically encoded far-red fluorescent indicator for monitoring synaptic Zn(2+) dynamics in the nervous system. Our engineered far-red fluorescent indicator for synaptic Zn(2+) (FRISZ) displayed a substantial Zn(2+)-specific turn-on response and low-micromolar affinity. We genetically anchored FRISZ to the mammalian extracellular membrane via a transmembrane (TM) helix and characterized the resultant FRISZ-TM construct at the mammalian cell surface. We used FRISZ-TM to image synaptic Zn(2+) in the auditory cortex in acute brain slices and awake mice in response to electric and sound stimuli, respectively. Thus, this study establishes a technology for studying the roles of synaptic Zn(2+) in the nervous system. |
