| First Author | Rodríguez-Tornos FM | Year | 2016 |
| Journal | Neuron | Volume | 89 |
| Issue | 3 | Pages | 494-506 |
| PubMed ID | 26804994 | Mgi Jnum | J:230869 |
| Mgi Id | MGI:5766400 | Doi | 10.1016/j.neuron.2015.12.020 |
| Citation | Rodriguez-Tornos FM, et al. (2016) Cux1 Enables Interhemispheric Connections of Layer II/III Neurons by Regulating Kv1-Dependent Firing. Neuron 89(3):494-506 |
| abstractText | Neuronal subtype-specific transcription factors (TFs) instruct key features of neuronal function and connectivity. Activity-dependent mechanisms also contribute to wiring and circuit assembly, but whether and how they relate to TF-directed neuronal differentiation is poorly investigated. Here we demonstrate that the TF Cux1 controls the formation of the layer II/III corpus callosum (CC) projections through the developmental transcriptional regulation of Kv1 voltage-dependent potassium channels and the resulting postnatal switch to a Kv1-dependent firing mode. Loss of Cux1 function led to a decrease in the expression of Kv1 transcripts, aberrant firing responses, and selective loss of CC contralateral innervation. Firing and innervation were rescued by re-expression of Kv1 or postnatal reactivation of Cux1. Knocking down Kv1 mimicked Cux1-mediated CC axonal loss. These findings reveal that activity-dependent processes are central bona fide components of neuronal TF-differentiation programs and establish the importance of intrinsic firing modes in circuit assembly within the neocortex. VIDEO ABSTRACT. |
