| First Author | Tao H | Year | 2020 |
| Journal | Development | Volume | 147 |
| Issue | 19 | PubMed ID | 32907847 |
| Mgi Jnum | J:297065 | Mgi Id | MGI:6471619 |
| Doi | 10.1242/dev.180042 | Citation | Tao H, et al. (2020) IRX3/5 regulate mitotic chromatid segregation and limb bud shape. Development 147(19):dev180042 |
| abstractText | Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation. |
