| First Author | Galbiati F | Year | 2000 |
| Journal | Eur J Immunol | Volume | 30 |
| Issue | 1 | Pages | 29-37 |
| PubMed ID | 10602024 | Mgi Jnum | J:59230 |
| Mgi Id | MGI:1351221 | Doi | 10.1002/1521-4141(200001)30:1<29::AID-IMMU29>3.0.CO;2-7 |
| Citation | Galbiati F, et al. (2000) IL-12 receptor regulation in IL-12-deficient BALB/c and C57BL/6 mice. Eur J Immunol 30(1):29-37 |
| abstractText | Immunization with protein antigen in complete Freund's adjuvant (CFA) induces Th1 cells in BALB/c and C57BL/6 (B6) mice. Pretreatment with the same protein in soluble form induces Th2 cells in BALB/c but not in B6 mice and inhibits Th1 cell development in both. We have previously shown that inhibition of Th1 in BALB/c mice correlates with the down-regulation of transcripts encoding the IL-12 receptor beta2 (IL-12Rbeta2) chain, which is required for IL-12 signaling and Th1 cell development. We now demonstrate that IL-12-deficient BALB/c mice, when primed with antigen in CFA, mount a Th2 instead of the Th1 response which develops in wild-type mice. Conversely, IL-12-deficient B6 mice fail to develop Th2 cells. Thus, a default Th2 development is induced by antigen priming in IL-12-deficient BALB/c but not B6 mice. IL-12Rbeta2 transcripts are still expressed in antigen-restimulated CD4(+) T cells from IL-12-deficient BALB/c and B6 mice and they are similarly reduced by pretreatment with soluble antigen, suggesting that intrinsic strain differences in IL-12R regulation do not account for the differential polarization to the Th2 pathway. IL-4 is not required for down-regulation of IL-12Rbeta2 transcripts and inhibition of Th1 development in mice pretreated with soluble protein, as shown by their reduction in IL-4-deficient BALB/c mice. |
