| First Author | Engler AE | Year | 2020 |
| Journal | Cell Rep | Volume | 33 |
| Issue | 13 | Pages | 108553 |
| PubMed ID | 33378665 | Mgi Jnum | J:304502 |
| Mgi Id | MGI:6514513 | Doi | 10.1016/j.celrep.2020.108553 |
| Citation | Engler AE, et al. (2020) Airway-Associated Macrophages in Homeostasis and Repair. Cell Rep 33(13):108553 |
| abstractText | There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells of the mouse trachea during homeostasis and epithelial injury/repair. We identify submucosal macrophages, similar to lung interstitial macrophages, and intraepithelial macrophages. Following injury, there are early increases in neutrophils and submucosal macrophages, including M2-like macrophages. Intraepithelial macrophages are lost after injury and later restored by CCR2(+) monocytes. We show that repair of the tracheal epithelium is impaired in Ccr2-deficient mice. Mast cells and group 2 innate lymphoid cells are sources of interleukin-13 (IL-13) that polarize macrophages and directly influence basal cell behaviors. Their proximity to the airway epithelium establishes these myeloid populations as potential therapeutic targets for airway disease. |
