| First Author | Sato-Hashimoto M | Year | 2011 |
| Journal | J Immunol | Volume | 187 |
| Issue | 1 | Pages | 291-7 |
| PubMed ID | 21632712 | Mgi Jnum | J:175930 |
| Mgi Id | MGI:5287943 | Doi | 10.4049/jimmunol.1100528 |
| Citation | Sato-Hashimoto M, et al. (2011) Signal regulatory protein alpha regulates the homeostasis of T lymphocytes in the spleen. J Immunol 187(1):291-7 |
| abstractText | The molecular basis for formation of lymphoid follicle and its homeostasis in the secondary lymphoid organs remains unclear. Signal regulatory protein alpha (SIRPalpha), an Ig superfamily protein that is predominantly expressed in dendritic cells or macrophages, mediates cell-cell signaling by interacting with CD47, another Ig superfamily protein. In this study, we show that the size of the T cell zone as well as the number of CD4(+) T cells were markedly reduced in the spleen of mice bearing a mutant (MT) SIRPalpha that lacks the cytoplasmic region compared with those of wild-type mice. In addition, the expression of CCL19 and CCL21, as well as of IL-7, which are thought to be important for development or homeostasis of the T cell zone, was markedly decreased in the spleen of SIRPalpha MT mice. By the use of bone marrow chimera, we found that hematopoietic SIRPalpha is important for development of the T cell zone as well as the expression of CCL19 and CCL21 in the spleen. The expression of lymphotoxin and its receptor, lymphotoxin beta receptor, as well as the in vivo response to lymphotoxin beta receptor stimulation were also decreased in the spleen of SIRPalpha MT mice. CD47-deficient mice also manifested phenotypes similar to SIRPalpha MT mice. These data suggest that SIRPalpha as well as its ligand CD47 are thus essential for steady-state homeostasis of T cells in the spleen. |
