| First Author | Lee LJ | Year | 2005 |
| Journal | J Neurosci | Volume | 25 |
| Issue | 9 | Pages | 2304-11 |
| PubMed ID | 15745956 | Mgi Jnum | J:98614 |
| Mgi Id | MGI:3579211 | Doi | 10.1523/JNEUROSCI.4902-04.2005 |
| Citation | Lee LJ, et al. (2005) NMDA receptor-dependent regulation of axonal and dendritic branching. J Neurosci 25(9):2304-11 |
| abstractText | In the rodent trigeminal principal nucleus (PrV), trigeminal afferent terminals and postsynaptic cells form discrete modules ('barrelettes') that replicate the patterned array of whiskers and sinus hairs on the snout. Barrelette neurons of the PrV relay whisker-specific patterns to the contralateral thalamus and, subsequently, to the primary somatosensory barrel cortex. Genetic impairment of NMDA receptor (NMDAR) function blocks development of barrelettes in the PrV. Underlying cellular and functional defects are not known. Here, we examined morphological differentiation of whisker afferents, dendritic differentiation of barrelette cells, and their electrophysiological properties in mice with genetic perturbations of the essential subunit NR1 of NMDARs. We show that in NR1 gene knock-down (KD) and knock-out mice, whisker afferents begin their embryonic development normally but, over time, fail to segregate into patches, and instead they develop exuberant terminal arbors spanning most of the PrV. Postnatal NR1KD barrelette cells, with significantly reduced NMDA currents, retain their membrane and synaptic properties but develop longer dendrites with no orientation preference. These results indicate that NMDARs regulate growth of presynaptic terminal arbors and postsynaptic dendritic branching, thereby leading to consolidation of synapses and patterning of presynaptic and postsynaptic elements. |
