| First Author | Shih TA | Year | 2002 |
| Journal | Nat Immunol | Volume | 3 |
| Issue | 4 | Pages | 399-406 |
| PubMed ID | 11896394 | Mgi Jnum | J:75653 |
| Mgi Id | MGI:2177332 | Doi | 10.1038/ni776 |
| Citation | Shih TA, et al. (2002) Role of antigen receptor affinity in T cell-independent antibody responses in vivo. Nat Immunol 3(4):399-406 |
| abstractText | To examine how B cell receptor affinity affects clonal selection in thymus-independent type 2 (TI-2) immune responses, we produced mice with antibodies that showed a 40-fold difference in affinity for the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). The difference in the responses of high- and low-affinity B cells to NP-Ficoll was only twofold. However, in competition experiments only the high-affinity B cells responded to antigen. CD19 deficiency increased the affinity threshold of TI-2 responses, whereas Lyn deficiency enhanced clonal expansion but abrogated B cell terminal differentiation. Thus, in TI-2 immune responses, large differences in affinity produce only small differences in the intrinsic ability of B cells to respond to antigen, and selection for high-affinity clones is due to clonal competition during the earliest stages of the response. |
