| First Author | Hikone K | Year | 2017 |
| Journal | J Histochem Cytochem | Volume | 65 |
| Issue | 4 | Pages | 207-221 |
| PubMed ID | 28122194 | Mgi Jnum | J:272255 |
| Mgi Id | MGI:6283807 | Doi | 10.1369/0022155416689670 |
| Citation | Hikone K, et al. (2017) Histochemical Examination on Periodontal Tissues of Klotho-Deficient Mice Fed With Phosphate-Insufficient Diet. J Histochem Cytochem 65(4):207-221 |
| abstractText | To elucidate which of elevated serum concentration of inorganic phosphate (Pi) or disrupted signaling linked to alphaklotho/fibroblast growth factor 23 (FGF23) is a predominant regulator for senescence-related degeneration seen in alphaKlotho-deficient mice, we have examined histological alteration of the periodontal tissues in the mandibular interalveolar septum of alphaKlotho-deficient mice fed with Pi-insufficient diet. We prepared six groups of mice: wild-type, kl/kl, and alphaKlotho(-/-) mice with normal diet or low-Pi diet. As a consequence, kl/kl(norPi) and alphaKlotho(-/-norPi) mice showed the same abnormalities in periodontal tissues: intensely stained areas with hematoxylin in the interalveolar septum, dispersed localization of alkaline phosphatase-positive osteoblasts and tartrate-resistant acid phosphatase-reactive osteoclasts, and accumulation of dentin matrix protein 1 in the osteocytic lacunae. Although kl/kl(lowPi) mice improved these histological abnormalities, alphaKlotho(-/- lowPi) mice failed to normalize those. Gene expression of alphaKlotho was shown to be increased in kl/kl (lowPi) specimens. It seems likely that histological abnormalities of kl/kl mice have been improved by the rescued expression of alphaKlotho, rather than low concentration of serum Pi. Thus, the histological malformation in periodontal tissues in alphaKlotho-deficient mice appears to be due to not only increased concentration of Pi but also disrupted alphaklotho/FGF23 signaling. |
