| First Author | Iwata H | Year | 2010 |
| Journal | Circulation | Volume | 122 |
| Issue | 20 | Pages | 2048-57 |
| PubMed ID | 21041690 | Mgi Jnum | J:179478 |
| Mgi Id | MGI:5302472 | Doi | 10.1161/CIRCULATIONAHA.110.965202 |
| Citation | Iwata H, et al. (2010) Bone marrow-derived cells contribute to vascular inflammation but do not differentiate into smooth muscle cell lineages. Circulation 122(20):2048-57 |
| abstractText | BACKGROUND: It has been proposed that bone marrow-derived cells infiltrate the neointima, where they differentiate into smooth muscle (SM) cells; however, technical limitations have hindered clear identification of the lineages of bone marrow-derived "SM cell-like" cells. METHODS AND RESULTS: Using a specific antibody against the definitive SM cell lineage marker SM myosin heavy chain (SM-MHC) and mouse lines in which reporter genes were driven by regulatory programs for either SM-MHC or SM alpha-actin, we demonstrated that although some bone marrow-derived cells express SM alpha-actin in the wire injury-induced neointima, those cells did not express SM-MHC, even 30 weeks after injury. Likewise, no SM-MHC(+) bone marrow-derived cells were found in vascular lesions in apolipoprotein E(-/-)mice or in a heart transplantation vasculopathy model. Instead, the majority of bone marrow-derived SM alpha-actin(+) cells were also CD115(+)CD11b(+)F4/80(+)Ly-6C(+), which is the surface phenotype of inflammatory monocytes. Moreover, adoptively transferred CD11b(+)Ly-6C(+) bone marrow cells expressed SM alpha-actin in the injured artery. Expression of inflammation-related genes was significantly higher in neointimal subregions rich in bone marrow-derived SM alpha-actin(+) cells than in other regions. CONCLUSIONS: It appears that bone marrow-derived SM alpha-actin(+) cells are of monocyte/macrophage lineage and are involved in vascular remodeling. It is very unlikely that these cells acquire the definitive SM cell lineage. |
