| First Author | Zhang N | Year | 2014 |
| Journal | Stem Cell Reports | Volume | 3 |
| Issue | 5 | Pages | 716-24 |
| PubMed ID | 25418719 | Mgi Jnum | J:289478 |
| Mgi Id | MGI:6436674 | Doi | 10.1016/j.stemcr.2014.09.012 |
| Citation | Zhang N, et al. (2014) ID1 is a functional marker for intestinal stem and progenitor cells required for normal response to injury. Stem Cell Reports 3(5):716-24 |
| abstractText | LGR5 and BMI1 mark intestinal stem cells in crypt base columnar cells and +4 position cells, respectively, but characterization of functional markers in these cell populations is limited. ID1 maintains the stem cell potential of embryonic, neural, and long-term repopulating hematopoietic stem cells. Here, we show in both human and mouse intestine that ID1 is expressed in cycling columnar cells, +4 position cells, and transit-amplifying cells in the crypt. Lineage tracing revealed ID1+ cells to be self-renewing, multipotent stem/progenitor cells that are responsible for the long-term renewal of the intestinal epithelium. Single ID1+ cells can generate long-lived organoids resembling mature intestinal epithelium. Complete knockout of Id1 or selective deletion of Id1 in intestinal epithelium or in LGR5+ stem cells sensitizes mice to chemical-induced colon injury. These experiments identify ID1 as a marker for intestinal stem/progenitor cells and demonstrate a role for ID1 in maintaining the potential for repair in response to colonic injury. |
