| First Author | Oommen S | Year | 2012 |
| Journal | Dev Dyn | Volume | 241 |
| Issue | 9 | Pages | 1465-72 |
| PubMed ID | 22753148 | Mgi Jnum | J:186496 |
| Mgi Id | MGI:5432441 | Doi | 10.1002/dvdy.23828 |
| Citation | Oommen S, et al. (2012) Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development. Dev Dyn 241(9):1465-72 |
| abstractText | Background: Tooth development is known to be mediated by the cross-talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19- to 25-nt noncoding small single-stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be important for the fine-tuning signaling pathways in development. To investigate the role of miRNAs in tooth development, we examined mice with either mesenchymal (Wnt1Cre/Dicer(fl/fl) ) or epithelial (ShhCre/Dicer(fl/fl) ) conditional deletion of Dicer, which is essential for miRNA processing. Results: By using a CD1 genetic background for Wnt1Cre/Dicer(fl/fl) , we were able to examine tooth development, because the mutants retained mandible and maxilla primordia. Wnt1Cre/Dicer(fl/fl) mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Extra incisor tooth formation was found in ShhCre/Dicer(fl/fl) mice, whereas molars showed no significant anomalies. Microarray and in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars. Conclusion: In tooth development, miRNAs thus play different roles in epithelium and mesenchyme, and in incisors and molars. Developmental Dynamics 241:1465-1472, 2012. (c) 2012 Wiley Periodicals, Inc. |
