| First Author | Anderson WJ | Year | 2008 |
| Journal | Dev Dyn | Volume | 237 |
| Issue | 2 | Pages | 504-12 |
| PubMed ID | 18213590 | Mgi Jnum | J:130142 |
| Mgi Id | MGI:3771102 | Doi | 10.1002/dvdy.21437 |
| Citation | Anderson WJ, et al. (2008) Genetic targeting of the endoderm with claudin-6(CreER). Dev Dyn 237(2):504-12 |
| abstractText | A full description of the ontogeny of the beta cell would guide efforts to generate beta cells from embryonic stem cells (ESCs). The first step requires an understanding of definitive endoderm: the genes and signals responsible for its specification, proliferation, and patterning. This report describes a global marker of definitive endoderm, Claudin-6 (Cldn6). We report its expression in early development with particular attention to definitive endoderm derivatives. To create a genetic system to drive gene expression throughout the definitive endoderm with both spatial and temporal control, we target the endogenous locus with an inducible Cre recombinase (Cre-ER(T2)) cassette. Cldn6 null mice are viable and fertile with no obvious phenotypic abnormalities. We also report a lineage analysis of the fate of Cldn6-expressing embryonic cells, which is relevant to the development of the pancreas, lung, and liver. Developmental Dynamics 237:504-512, 2008. (c) 2008 Wiley-Liss, Inc. |
