| First Author | Mullican SE | Year | 2013 |
| Journal | Mol Endocrinol | Volume | 27 |
| Issue | 1 | Pages | 127-34 |
| PubMed ID | 23192980 | Mgi Jnum | J:199860 |
| Mgi Id | MGI:5505675 | Doi | 10.1210/me.2012-1267 |
| Citation | Mullican SE, et al. (2013) A novel adipose-specific gene deletion model demonstrates potential pitfalls of existing methods. Mol Endocrinol 27(1):127-34 |
| abstractText | Adipose-specific gene deletion in mice is crucial in determining gene function in adipocyte homeostasis and the development of obesity. We noted 100% mortality when the Hdac3 gene was conditionally deleted using Fabp4-Cre mice, the most commonly used model of adipose-targeted Cre recombinase. However, this surprising result was not reproduced using other models of adipose targeting of Cre, including a novel Retn-Cre mouse. These findings underscore the need for caution when interpreting data obtained using Fabp4-Cre mice and should encourage the use of additional or alternative adipose-targeting Cre mouse models before drawing conclusions about in vivo adipocyte-specific functions. |
