| First Author | Muncan V | Year | 2006 |
| Journal | Mol Cell Biol | Volume | 26 |
| Issue | 22 | Pages | 8418-26 |
| PubMed ID | 16954380 | Mgi Jnum | J:114673 |
| Mgi Id | MGI:3689687 | Doi | 10.1128/MCB.00821-06 |
| Citation | Muncan V, et al. (2006) Rapid Loss of Intestinal Crypts upon Conditional Deletion of the Wnt/Tcf-4 Target Gene c-Myc. Mol Cell Biol 26(22):8418-26 |
| abstractText | Inhibition of the mutationally activated Wnt cascade in colorectal cancer cell lines induces a rapid G(1) arrest and subsequent differentiation. This arrest can be overcome by maintaining expression of a single Tcf4 target gene, the proto-oncogene c-Myc. Since colorectal cancer cells share many molecular characteristics with proliferative crypt progenitors, we have assessed the physiological role of c-Myc in adult crypts by conditional gene deletion. c-Myc-deficient crypts are lost within weeks and replaced by c-Myc-proficient crypts through a fission process of crypts that have escaped gene deletion. Although c-Myc(-)(/)(-) crypt cells remain in the cell cycle, they are on average much smaller than wild-type cells, cycle slower, and divide at a smaller cell size. c-Myc appears essential for crypt progenitor cells to provide the necessary biosynthetic capacity to successfully progress through the cell cycle. |
