| First Author | Pérez-Millán MI | Year | 2013 |
| Journal | Genesis | Volume | 51 |
| Issue | 11 | Pages | 785-92 |
| PubMed ID | 23996951 | Mgi Jnum | J:204533 |
| Mgi Id | MGI:5532776 | Doi | 10.1002/dvg.22425 |
| Citation | Perez-Millan MI, et al. (2013) Efficient, specific, developmentally appropriate cre-mediated recombination in anterior pituitary gonadotropes and thyrotropes. Genesis 51(11):785-92 |
| abstractText | Tissue-specific expression of cre recombinase is a well-established genetic tool to analyze gene function, and it is limited only by the efficiency and specificity of available cre mouse strains. Here, we report the generation of a transgenic line containing a cre cassette with codon usage optimized for mammalian cells (iCre) under the control of a mouse glycoprotein hormone alpha-subunit (alphaGSU) regulatory sequences in a bacterial artificial chromosome genomic clone. Initial analysis of this transgenic line, Tg(alphaGSU-iCre), with cre reporter strains reveals onset of cre activity in the differentiating cells of the developing anterior pituitary gland at embryonic day 12.5, with a pattern characteristic of endogenous alphaGSU. In adult mice, alphaGSU-iCre was active in the anterior lobe of the pituitary gland and in the cells that produce alphaGSU (gonadotropes and thyrotropes) with high penetrance. Little or no activity was observed in other tissues, including skeletal and cardiac muscle, brain, kidney, lungs, testis, ovary, and liver. This alphaGSU-iCre line is suitable for efficient, specific, and developmentally regulated deletion of floxed alleles in anterior pituitary gonadotropes and thyrotropes. |
