| First Author | Ding G | Year | 2013 |
| Journal | Dev Dyn | Volume | 242 |
| Issue | 3 | Pages | 254-68 |
| PubMed ID | 23335233 | Mgi Jnum | J:193389 |
| Mgi Id | MGI:5468359 | Doi | 10.1002/dvdy.23923 |
| Citation | Ding G, et al. (2013) PDGF Receptor Alpha+ Mesoderm Contributes to Endothelial and Hematopoietic Cells in Mice. Dev Dyn 242(3):254-68 |
| abstractText | Background: Early mesoderm can be classified into Flk-1+ or PDGF receptor alpha (PDGFRalpha)+ population, grossly representing lateral and paraxial mesoderm, respectively. It has been demonstrated that all endothelial (EC) and hematopoietic (HPC) cells are derived from Flk-1+ cells. Although PDGFRalpha+ cells give rise to ECs/HPCs in in vitro ES differentiation, whether PDGFRalpha+ population can become hemato-endothelial lineages has not been proved in mouse embryos. Results: Using PDGFRalphaMerCreMer mice, PDGFRalpha+ early mesoderm was shown to contribute to endothelial cells including hemogenic ECs, fetal liver B lymphocytes, and Lin-Kit+Sca-1+ (KSL) cells. Contribution of PDGFRalpha+ mesoderm into ECs and HPCs was limited until E8.5, indicating that PDGFRalpha+/Flk-1+ population that exists until E8.5 may be the source for hemato-endothelial lineages from PDGFRalpha+ population. The functional significance of PDGFRalpha+ mesoderm in vascular development and hematopoiesis was confirmed by genetic deletion of Etv2 or restoration of Runx1 in PDGFRalpha+ cells. Etv2 deletion and Runx1 restoration in PDGFRalpha+ cells resulted in abnormal vascular remodeling and rescue of fetal liver CD45+ and Lin-Kit+Sca-1+ (KSL) cells, respectively. Conclusions: Endothelial and hematopoietic cells can be derived from PDGFRalpha+ early mesoderm in mice. PDGFRalpha+ mesoderm is functionally significant in vascular development and hematopoiesis from phenotype analysis of genetically modified embryos. Developmental Dynamics 242:254-268, 2013. (c) 2013 Wiley Periodicals, Inc. |
