| First Author | Schmitz F | Year | 2017 |
| Journal | Genesis | Volume | 55 |
| Issue | 11 | PubMed ID | 28948682 |
| Mgi Jnum | J:254725 | Mgi Id | MGI:6098772 |
| Doi | 10.1002/dvg.23073 | Citation | Schmitz F, et al. (2017) A novel Cre-inducible knock-in ARL13B-tRFP fusion cilium reporter. Genesis 55(11) |
| abstractText | Cilia play a major role in the regulation of numerous signaling pathways and are essential for embryonic development. Mutations in genes affecting ciliary function can cause a variety of diseases in humans summarized as ciliopathies. To facilitate the detection and visualization of cilia in a temporal and spatial manner in mouse tissues, we generated a Cre-inducible cilium-specific reporter mouse line expressing an ARL13B-tRFP fusion protein driven by a CMV enhancer/chicken beta actin promotor (pCAG) from the Hprt locus. We detected bright and specific ciliary signals by immunostainings of various mono- and multiciliated tissues and by time-lapse live-cell analysis of cultured embryos and organ explant cultures. Additionally, we monitored cilium assembly and disassembly in embryonic fibroblast cells using live-cell imaging. Thus, the ARL13B-tRFP reporter mouse strain is a valuable tool for the investigation of ciliary structure and function in a tissue-specific manner to understand processes, such as ciliary protein trafficking or cilium-dependent signaling in vitro and in vivo. |
