| First Author | Brewster JL | Year | 2000 |
| Journal | Genesis | Volume | 26 |
| Issue | 4 | Pages | 271-8 |
| PubMed ID | 10748466 | Mgi Jnum | J:62083 |
| Mgi Id | MGI:1858316 | Citation | Brewster JL, et al. (2000) Deletion of Dad1 in mice induces an apoptosis-associated embryonic death. Genesis 26(4):271-8 |
| abstractText | Dad1 is a putative anti-apoptosis gene identified in several distantly related organisms. Expression of Dad1 in transfected cells inhibits apoptosis in vitro. To determine whether Dad1 has a similar function in vivo, we used gene targeting to delete Dad1. Heterozygous adult mice (+/-) show no obvious phenotype or abnormalities, but genotype analysis of over 100 offspring from heterozygous matings detected no weanling, homozygous Dad1 null (-/-) mice. Subsequent analysis of embryos from heterozygous matings detected Dad1 null (-/-) embryos at E3.5 but no later, suggesting Dad1 is required for development beyond the late blastocyst stage. Increased levels of apoptosis were observed in cultured embryos lacking a functional copy of the gene, consistent with an anti-apoptotic role for Dad1. |
