| First Author | Nishimura T | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 323 |
| Issue | 1 | Pages | 218-22 |
| PubMed ID | 15351724 | Mgi Jnum | J:92537 |
| Mgi Id | MGI:3053477 | Doi | 10.1016/j.bbrc.2004.08.087 |
| Citation | Nishimura T, et al. (2004) Cellular prion protein regulates intracellular hydrogen peroxide level and prevents copper-induced apoptosis. Biochem Biophys Res Commun 323(1):218-22 |
| abstractText | The function of cellular prion protein (PrP(C)), which is a copper binding protein, remains unclear. To elucidate the mechanisms in which PrP(C) is involved in neuroprotection, we compared death signals in prion protein gene-deficient (Prnp(-/-)) primary cerebellar granular neurons (CGNs) to those with wild-type (WT) CGNs. When copper was exposed to these CGNs, ZrchI, and Rikn Prnp(-/-) CGNs were more sensitized and underwent apoptotic cell death more readily than WT CGNs. Furthermore, the level of intracellular hydrogen peroxide (H(2)O(2)) in WT CGNs increased by copper toxicity, whereas those in ZrchI and Rikn Prnp(-/-) CGNs did not. These results suggest that PrP(C) modulates the intracellular H(2)O(2) level as a copper-binding protein to protect CGNs from apoptotic cell death possibly due to inhibiting a Fenton reaction. |
