| First Author | Gomi H | Year | 2021 |
| Journal | J Histochem Cytochem | Volume | 69 |
| Issue | 4 | Pages | 229-243 |
| PubMed ID | 33622062 | Mgi Jnum | J:331523 |
| Mgi Id | MGI:7386964 | Doi | 10.1369/0022155421996845 |
| Citation | Gomi H, et al. (2021) Expression Pattern of the LacZ Reporter in Secretogranin III Gene-trapped Mice. J Histochem Cytochem 69(4):229-243 |
| abstractText | Secretogranin III (SgIII) is a granin protein involved in secretory granule formation in peptide-hormone-producing endocrine cells. In this study, we analyzed the expression of the LacZ reporter in the SgIII knockout mice produced by gene trapping (SgIII-gtKO) for the purpose of comprehensively clarifying the expression patterns of SgIII at the cell and tissue levels. In the endocrine tissues of SgIII-gtKO mice, LacZ expression was observed in the pituitary gland, adrenal medulla, and pancreatic islets, where SgIII expression has been canonically revealed. LacZ expression was extensively observed in brain regions, especially in the cerebral cortex, hippocampus, hypothalamic nuclei, cerebellum, and spinal cord. In peripheral nervous tissues, LacZ expression was observed in the retina, optic nerve, and trigeminal ganglion. LacZ expression was particularly prominent in astrocytes, in addition to neurons and ependymal cells. In the cerebellum, at least four cell types expressed SgIII under basal conditions. The expression of SgIII in the glioma cell lines C6 and RGC-6 was enhanced by excitatory glutamate treatment. It also became clear that the expression level of SgIII varied among neuron and astrocyte subtypes. These results suggest that SgIII is involved in glial cell function, in addition to neuroendocrine functions, in the nervous system. |
