| First Author | Arai M | Year | 2024 |
| Journal | Biochem Biophys Res Commun | Volume | 692 |
| Pages | 149364 | PubMed ID | 38070276 |
| Mgi Jnum | J:343771 | Mgi Id | MGI:7569930 |
| Doi | 10.1016/j.bbrc.2023.149364 | Citation | Arai M, et al. (2023) Effect of Sparc knockout on the extracellular matrix of mouse periodontal ligament cells. Biochem Biophys Res Commun 692:149364 |
| abstractText | The periodontal ligament (PDL) is a critical component in maintaining tooth stability. It is composed of cells and an extracellular matrix (ECM), each with unique roles in tissue function and homeostasis. Secreted protein acidic and rich in cysteine (SPARC), a calcium-binding matricellular glycoprotein, plays a crucial role in regulating ECM assembly and turnover, alongside facilitating cellular-ECM interactions. In the present study, mass spectrometry-based proteomics was used to assess the impacts of Sparc-knockout (KO) on PDL-derived cells. Results demonstrated that Sparc-KO significantly reduces ECM production and alters its composition with increased levels of type I collagen. Despite this increase in Sparc-KO, type I collagen was not likely to be effectively integrated into the fibrils due to collagen cross-linking impairment. Furthermore, the pathway and process enrichment analyses suggested that SPARC plays a protective role against ECM degradation by antagonistically interacting with cell-surface collagen receptors. These findings provide detailed insights into the multifaceted role of SPARC in ECM organization, including its impact on ECM production, collagen regulation, and interactions with various cellular compartments. A better understanding of these complex mechanisms is crucial for comprehending the causes of periodontal disease and tissue regeneration, where precise control of ECM organization is necessary. |
