| First Author | Elias BC | Year | 2014 |
| Journal | J Biol Chem | Volume | 289 |
| Issue | 12 | Pages | 8532-44 |
| PubMed ID | 24509849 | Mgi Jnum | J:212444 |
| Mgi Id | MGI:5581401 | Doi | 10.1074/jbc.M113.526509 |
| Citation | Elias BC, et al. (2014) The integrin beta1 subunit regulates paracellular permeability of kidney proximal tubule cells. J Biol Chem 289(12):8532-44 |
| abstractText | Epithelial cells lining the gastrointestinal tract and kidney have different abilities to facilitate paracellular and transcellular transport of water and solutes. In the kidney, the proximal tubule allows both transcellular and paracellular transport, while the collecting duct primarily facilitates transcellular transport. The claudins and E-cadherin are major structural and functional components regulating paracellular transport. In this study we present the novel finding that the transmembrane matrix receptors, integrins, play a role in regulating paracellular transport of renal proximal tubule cells. Deleting the integrin beta1 subunit in these cells converts them from a "loose" epithelium, characterized by low expression of E-cadherin and claudin-7 and high expression of claudin-2, to a "tight" epithelium with increased E-cadherin and claudin-7 expression and decreased claudin-2 expression. This effect is mediated by the integrin beta1 cytoplasmic tail and does not entail beta1 heterodimerization with an alpha-subunit or its localization to the cell surface. In addition, we demonstrate that deleting the beta1 subunit in the proximal tubule of the kidney results in a major urine-concentrating defect. Thus, the integrin beta1 tail plays a key role in regulating the composition and function of tight and adherens junctions that define paracellular transport properties of terminally differentiated renal proximal tubule epithelial cells. |
