| First Author | Seo GY | Year | 2022 |
| Journal | Sci Immunol | Volume | 7 |
| Issue | 73 | Pages | eabm6931 |
| PubMed ID | 35905286 | Mgi Jnum | J:337669 |
| Mgi Id | MGI:7493575 | Doi | 10.1126/sciimmunol.abm6931 |
| Citation | Seo GY, et al. (2022) Epithelial HVEM maintains intraepithelial T cell survival and contributes to host protection. Sci Immunol 7(73):eabm6931 |
| abstractText | Intraepithelial T cells (IETs) are in close contact with intestinal epithelial cells and the underlying basement membrane, and they detect invasive pathogens. How intestinal epithelial cells and basement membrane influence IET survival and function, at steady state or after infection, is unclear. The herpes virus entry mediator (HVEM), a member of the TNF receptor superfamily, is constitutively expressed by intestinal epithelial cells and is important for protection from pathogenic bacteria. Here, we showed that at steady-state LIGHT, an HVEM ligand, binding to epithelial HVEM promoted the survival of small intestine IETs. RNA-seq and addition of HVEM ligands to epithelial organoids indicated that HVEM increased epithelial synthesis of basement membrane proteins, including collagen IV, which bound to beta(1) integrins expressed by IETs. Therefore, we proposed that IET survival depended on beta(1) integrin binding to collagen IV and showed that beta(1) integrin-collagen IV interactions supported IET survival in vitro. Moreover, the absence of beta(1) integrin expression by T lymphocytes decreased TCR alphabeta(+) IETs in vivo. Intravital microscopy showed that the patrolling movement of IETs was reduced without epithelial HVEM. As likely consequences of decreased number and movement, protective responses to Salmonella enterica were reduced in mice lacking either epithelial HVEM, HVEM ligands, or beta(1) integrins. Therefore, IETs, at steady state and after infection, depended on HVEM expressed by epithelial cells for the synthesis of collagen IV by epithelial cells. Collagen IV engaged beta(1) integrins on IETs that were important for their maintenance and for their protective function in mucosal immunity. |
