| First Author | Saito K | Year | 2015 |
| Journal | PLoS One | Volume | 10 |
| Issue | 4 | Pages | e0121667 |
| PubMed ID | 25830530 | Mgi Jnum | J:233429 |
| Mgi Id | MGI:5784621 | Doi | 10.1371/journal.pone.0121667 |
| Citation | Saito K, et al. (2015) Interaction between fibronectin and beta1 integrin is essential for tooth development. PLoS One 10(4):e0121667 |
| abstractText | The dental epithelium and extracellular matrix interact to ensure that cell growth and differentiation lead to the formation of teeth of appropriate size and quality. To determine the role of fibronectin in differentiation of the dental epithelium and tooth formation, we analyzed its expression in developing incisors. Fibronectin mRNA was expressed during the presecretory stage in developing dental epithelium, decreased in the secretory and early maturation stages, and then reappeared during the late maturation stage. The binding of dental epithelial cells derived from postnatal day-1 molars to a fibronectin-coated dish was inhibited by the RGD but not RAD peptide, and by a beta1 integrin-neutralizing antibody, suggesting that fibronectin-beta1 integrin interactions contribute to dental epithelial-cell binding. Because fibronectin and beta1 integrin are highly expressed in the dental mesenchyme, it is difficult to determine precisely how their interactions influence dental epithelial differentiation in vivo. Therefore, we analyzed beta1 integrin conditional knockout mice (Intbeta1lox-/lox-/K14-Cre) and found that they exhibited partial enamel hypoplasia, and delayed eruption of molars and differentiation of ameloblasts, but not of odontoblasts. Furthermore, a cyst-like structure was observed during late ameloblast maturation. Dental epithelial cells from knockout mice did not bind to fibronectin, and induction of ameloblastin expression in these cells by neurotrophic factor-4 was inhibited by treatment with RGD peptide or a fibronectin siRNA, suggesting that the epithelial interaction between fibronectin and beta1 integrin is important for ameloblast differentiation and enamel formation. |
