| Year | 2000 | Journal | J Invest Dermatol |
| Volume | 114 | Issue | 4 |
| Pages | 747-888 | PubMed ID | 10745038 |
| Mgi Jnum | J:65042 | Mgi Id | MGI:1891616 |
| Doi | 10.1046/j.1523-1747.2002.01194.x | Citation | Uyttendaele H, et al. (2000) Abstracts for the 61st annual meeting of the society for investigative dermatology (Targeted overexpression of Notch1 in the hair follicle leads to defects in inner root sheath differentiation and dysregulation of hair cycling). J Invest Dermatol 114:747-888 (754 (Abstr)) |
| abstractText | Full text of Abstract: ABSTRACTS OF THE 61ST ANNUAL MEETING OF THE SOCIETY FOR INVESTIGATIVE DERMATOLOGY. Abstract Number: 030. Abstract Title: Targeted Overexpression of Notch 1 in the Hair Follicle Leads to Defects in Inner Root Sheath Differentiation and Dysregulation of Hair Cycling Author(s): H. Uyttendaele, V.M. Aita, J. Kitalewski, A.A. Panteleyev and : A.M. Christiano. Columbia University, New York, New York. Abstract: Notch genes encode a family of highly conserved transmembrane receptor proteins that play a pivotal role in cell fate decisions during development. Several Notch genes and their corresponding ligands are expressed in the developing hair follicle. We have used a transgenic approach to investigate the role of Notch1 on hair follicle development. A gain-of-function mutation was engineered using the involucrin promoter to drive expression of Notch1 in the inner root sheath (IRS). To facilitate analysis of transgene expression, beta-galactosidase expression was driven concurrently by the involucrin promoter using an internal ribosomal entry site (IRES). Further, the Notch1 transgene was epitope tagged in order to distinguish transgenic from endogenous Notchl protein expression in immunohistochemistry and western blotting. Several founder mice were generated and stable transgenic lines were established that express the Notch 1 transgene at different levels. Coats of transgenic animals display an overall disheveled appearance with wavy hairs and short, curly vibrissae and a diffuse pattern of alopecia. Additionally, mice demonstrate temporal abnormalities throughout the hair cycle, both within and between hair follicles. Histological examination reveals a primary defect in differentiation of the IRS, persistence of IRS remnants in later stages of the hair cycle, and loose contact between the ORS and hair shaft. These findings provide evidence supporting a role for Notchl in the regulation of cell fate decisions during hair follicle development. |
