| First Author | Horikawa K | Year | 1999 |
| Journal | Int Immunol | Volume | 11 |
| Issue | 9 | Pages | 1441-9 |
| PubMed ID | 10464165 | Mgi Jnum | J:57707 |
| Mgi Id | MGI:1345567 | Doi | 10.1093/intimm/11.9.1441 |
| Citation | Horikawa K, et al. (1999) Distinctive roles of Fyn and Lyn in IgD- and IgM-mediated signaling. Int Immunol 11(9):1441-9 |
| abstractText | Src family kinases Fyn and Lyn associate with the B cell antigen receptor (BCR). Accumulating data show that Lyn plays important roles in BCR-mediated signaling, while the role of Fyn remains obscure. Here we dissected the role of Fyn and Lyn in BCR signaling using B cells from fyn(-/-), lyn(-/-) and fyn/lyn double-deficient (fyn(-/-)lyn(-/-)) mice. In contrast to previous reports, fyn(-/-) B cells were slightly hyporeactive to both anti-IgM and anti-IgD-dextran. Although lyn(-/-) B cells were hyper-reactive to anti-IgM, anti-IgD-induced proliferation was impaired in lyn(-/-) B cells. Most of the other phenotypes of fyn(-/-)lyn(-/-) mice were similar to that of lyn(-/-) mice, except that proliferative responses of B cells to various stimuli, such as BCR cross-linking and lipopolysaccharide, were significantly lower in fyn(-/-)lyn(-/-) mice than in lyn(-/-) mice. Finally, immune responses to thymus-independent type 2 antigen were affected in these mutant mice. These observations suggest that Fyn and Lyn are involved in B cell functions, and play similar, but partly distinct, roles in BCR signaling. |
