| First Author | Clark H | Year | 2002 |
| Journal | J Immunol | Volume | 169 |
| Issue | 6 | Pages | 2892-9 |
| PubMed ID | 12218102 | Mgi Jnum | J:111599 |
| Mgi Id | MGI:3654565 | Doi | 10.4049/jimmunol.169.6.2892 |
| Citation | Clark H, et al. (2002) Surfactant protein D reduces alveolar macrophage apoptosis in vivo. J Immunol 169(6):2892-9 |
| abstractText | Surfactant protein D (SP-D) is a molecule of the innate immune system that recognizes the patterns of surface carbohydrate on pathogens and targets them for phagocytosis and killing. SP-D-deficient mice show an increased number of macrophages in the alveolar space, excess surfactant phospholipid, overproduction of reactive oxygen species, and the development of emphysema. We report here that SP-D-deficient mice have a 5- to 10-fold increase in the number of apoptotic and necrotic alveolar macrophages, as defined by annexin V and propidium iodine staining, respectively. Intrapulmonary administration of a truncated 60-kDa fragment of human recombinant SP-D reduces the number of apoptotic and necrotic alveolar macrophages and partially corrects the lipid accumulation in SP-D-deficient mice. The same SP-D fragment binds preferentially to apoptotic and necrotic alveolar macrophages in vitro, suggesting that SP-D contributes to immune homeostasis in the lung by recognizing and promoting removal of necrotic and apoptotic cells. |
