| First Author | Qin Y | Year | 2021 |
| Journal | Dev Biol | Volume | 477 |
| Pages | 232-240 | PubMed ID | 34097879 |
| Mgi Jnum | J:332706 | Mgi Id | MGI:6759667 |
| Doi | 10.1016/j.ydbio.2021.05.020 | Citation | Qin Y, et al. (2021) Nkx2-2 expressing taste cells in endoderm-derived taste papillae are committed to the type III lineage. Dev Biol 477:232-240 |
| abstractText | In mammals, multiple cell-signaling pathways and transcription factors regulate development of the embryonic taste system and turnover of taste cells in the adult stage. Using single-cell RNA-Seq of mouse taste cells, we found that the homeobox-containing transcription factor Nkx2-2, a target of the Sonic Hedgehog pathway and a key regulator of the development and regeneration of multiple cell types in the body, is highly expressed in type III taste cells but not in type II or taste stem cells. Using in situ hybridization and immunostaining, we confirmed that Nkx2-2 is expressed specifically in type III taste cells in the endoderm-derived circumvallate and foliate taste papillae but not in the ectoderm-derived fungiform papillae. Lineage tracing revealed that Nkx2-2-expressing cells differentiate into type III, but not type II or type I cells in circumvallate and foliate papillae. Neonatal Nkx2-2-knockout mice did not express key type III taste cell marker genes, while the expression of type II and type I taste cell marker genes were unaffected in these mice. Our findings indicate that Nkx2-2-expressing cells are committed to the type III lineage and that Nkx2-2 may be critical for the development of type III taste cells in the posterior tongue, thus illustrating a key difference in the mechanism of type III cell lineage specification between ectoderm- and endoderm-derived taste fields. |
