| First Author | Aden K | Year | 2019 |
| Journal | Gastroenterology | Volume | 156 |
| Issue | 1 | Pages | 145-159.e19 |
| PubMed ID | 30273559 | Mgi Jnum | J:278369 |
| Mgi Id | MGI:6323303 | Doi | 10.1053/j.gastro.2018.09.047: |
| Citation | Aden K, et al. (2019) Epithelial RNase H2 Maintains Genome Integrity and Prevents Intestinal Tumorigenesis in Mice. Gastroenterology 156(1):145-159.e19 |
| abstractText | BACKGROUND & AIMS: RNase H2 is a holoenzyme, composed of 3 subunits (ribonuclease H2 subunits A, B, and C), that cleaves RNA:DNA hybrids and removes mis-incorporated ribonucleotides from genomic DNA through ribonucleotide excision repair. Ribonucleotide incorporation by eukaryotic DNA polymerases occurs during every round of genome duplication and produces the most frequent type of naturally occurring DNA lesion. We investigated whether intestinal epithelial proliferation requires RNase H2 function and whether RNase H2 activity is disrupted during intestinal carcinogenesis. METHODS: We generated mice with epithelial-specific deletion of ribonuclease H2 subunit B (H2b(DeltaIEC)) and mice that also had deletion of tumor-suppressor protein p53 (H2b/p53(DeltaIEC)); we compared phenotypes with those of littermate H2b(fl/fl) or H2b/p53(fl/fl) (control) mice at young and old ages. Intestinal tissues were collected and analyzed by histology. We isolated epithelial cells, generated intestinal organoids, and performed RNA sequence analyses. Mutation signatures of spontaneous tumors from H2b/p53(DeltaIEC) mice were characterized by exome sequencing. We collected colorectal tumor specimens from 467 patients, measured levels of ribonuclease H2 subunit B, and associated these with patient survival times and transcriptome data. RESULTS: The H2b(DeltaIEC) mice had DNA damage to intestinal epithelial cells and proliferative exhaustion of the intestinal stem cell compartment compared with controls and H2b/p53(DeltaIEC) mice. However, H2b/p53(DeltaIEC) mice spontaneously developed small intestine and colon carcinomas. DNA from these tumors contained T>G base substitutions at GTG trinucleotides. Analyses of transcriptomes of human colorectal tumors associated lower levels of RNase H2 with shorter survival times. CONCLUSIONS: In analyses of mice with disruption of the ribonuclease H2 subunit B gene and colorectal tumors from patients, we provide evidence that RNase H2 functions as a colorectal tumor suppressor. H2b/p53(DeltaIEC) mice can be used to study the roles of RNase H2 in tissue-specific carcinogenesis. |
