| First Author | Wei LN | Year | 1996 |
| Journal | J Biol Chem | Volume | 271 |
| Issue | 9 | Pages | 5073-8 |
| PubMed ID | 8617785 | Mgi Jnum | J:32048 |
| Mgi Id | MGI:79551 | Doi | 10.1074/jbc.271.9.5073 |
| Citation | Wei LN, et al. (1996) Promoter and upstream regulatory activities of the mouse cellular retinoic acid-binding protein-I gene. J Biol Chem 271(9):5073-8 |
| abstractText | The promoter and its upstream regulatory region of the mouse cellular retinoic acid-binding protein I (crabp-I) gene were examined in transgenic mouse embryos, a mouse embryonal carcinoma cell line P19, and a mouse embryonic fibroblast cell line 3T6. In transgenic mouse embryos, a beta-galactosidase reporter gene under the control of crabp-I promoter and its upstream regulatory region displayed a very specific pattern of expression characteristic of crabp-I gene expression during developmental stages. In tissue culture systems, the minimal promoter of this gene was identified, and regions containing positive and negative regulatory activities were dissected from the upstream 3-kilobase sequence using assays for transient reporter activity. It is concluded that the minimal promoter of the mouse crabp-I gene is located between 120 and 150 base pairs upstream from the transcription initiation site. Several cell type-specific positive and negative regulatory regions for this promoter have been identified. A region encoding a common negative regulatory activity in both P19 and 3T6 cells is also inhibitory to two heterologous promoters, and specific protein-DNA interactions between this DNA fragment and nuclear extracts of P19 and 3T6 are demonstrated by gel retardation experiments. |
