| First Author | Wu Y | Year | 2021 |
| Journal | Biol Reprod | Volume | 104 |
| Issue | 3 | Pages | 539-547 |
| PubMed ID | 33284968 | Mgi Jnum | J:303812 |
| Mgi Id | MGI:6510986 | Doi | 10.1093/biolre/ioaa222 |
| Citation | Wu Y, et al. (2021) Notch1 is crucial for decidualization and maintaining the first pregnancy in the mouse. Biol Reprod 104(3):539-547 |
| abstractText | The endometrium undergoes a pregnancy-delivery-repair cycle multiple times during the reproductive lifespan in females. Decidualization is one of the critical events for the success of this essential process. We have previously reported that Notch1 is essential for artificial decidualization in mice. However, in a natural pregnancy, the deletion of Notch1 (PgrCre/+Notch1f/f, or Notch1d/d) only affects female fertility in the first 30 days of a 6-month fertility test, but not the later stages. In the present study, we undertook a closer evaluation at the first pregnancy of these mice to attempt to understand this puzzling phenomenon. We observed a large number of pregnancy losses in Notch1d/d mice in their first pregnancy, which led to the subfertility observed in the first 30 days of the fertility test. We then demonstrated that the initial pregnancy loss is a consequence of impaired decidualization. Furthermore, we identified a group of genes that contribute to Notch1 regulated decidualization in a natural pregnancy. Gene ontogeny analysis showed that these differentially expressed genes in the natural pregnancy are involved in cell-cell and cell-matrix interactions, different from genes that have been previously identified from the artificial decidualization model, which contribute to cell proliferation and apoptosis. In summary, we determined that Notch1 is essential for normal decidualization in the mouse uterus only in the first pregnancy but not in subsequent ones. |
