| Primary Identifier | MGI:1934021 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Prl |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A PGK neomycin cassette was inserted into exon 4. This sequence encodes the second alpha helix of the protein. RT-PCR analysis confirmed a lack of wild-type transcript in homozygous mutant mice. Western blotting of pituitary gland extracts revealed the presence of a unique immunoreactive ~10-kDa truncated protein product in homozygous mutant mice. Disrupted prolactin synthesis was confirmed by biological assays. |
