| First Author | Kilpatrick AM | Year | 2023 |
| Journal | STAR Protoc | Volume | 4 |
| Issue | 1 | Pages | 102016 |
| PubMed ID | 36640365 | Mgi Jnum | J:356173 |
| Mgi Id | MGI:7762265 | Doi | 10.1016/j.xpro.2022.102016 |
| Citation | Kilpatrick AM, et al. (2023) Protocol to analyze and validate transcriptomic changes in PDGFRbeta-KO mesenchymal stem cell osteogenic potential in the mouse embryo. STAR Protoc 4(1):102016 |
| abstractText | Mesenchymal stem/stromal cells (MSCs) can differentiate into osteoblasts under appropriate conditions. PDGFRbeta signaling controls MSC osteogenic potential both transcriptomically and in culture. Here, we present a "computer to the bench" protocol to analyze changes in MSC osteogenic potential at transcriptomic and cellular level in the absence of PDGFRbeta. We detail the preparation of cells from mouse embryos, the analysis of transcriptomic changes from single-cell RNA-sequencing data, the procedure for MSC derivation and culture, and an osteogenic assay for functional validation. For complete details on the use and execution of this protocol, please refer to Sa da Bandeira et al. (2022).(1). |
