| First Author | Khandelwal PJ | Year | 2011 |
| Journal | Hum Mol Genet | Volume | 20 |
| Issue | 11 | Pages | 2091-102 |
| PubMed ID | 21378096 | Mgi Jnum | J:171507 |
| Mgi Id | MGI:4950302 | Doi | 10.1093/hmg/ddr091 |
| Citation | Khandelwal PJ, et al. (2011) Parkin mediates beclin-dependent autophagic clearance of defective mitochondria and ubiquitinated A{beta} in AD models. Hum Mol Genet 20(11):2091-102 |
| abstractText | Intraneuronal amyloid-beta (Abeta) may contribute to extracellular plaque deposition, the characteristic pathology of Alzheimer's disease (AD). The E3-ubiquitin ligase parkin ubiquitinates intracellular proteins and induces mitophagy. We previously demonstrated that parkin reduces Abeta levels in lentiviral models of intracellular Abeta. Here we used a triple transgenic AD (3xTg-AD) mouse, which over-expresses APP(Swe), Tau(P301L) and harbor the PS1(M146V) knock-in mutation and found that lentiviral parkin ubiquitinated intracellular Abeta in vivo, stimulated beclin-dependent molecular cascade of autophagy and facilitated clearance of vesicles containing debris and defective mitochondria. Parkin expression decreased intracellular Abeta levels and extracellular plaque deposition. Parkin expression also attenuated caspase activity, prevented mitochondrial dysfunction and oxidative stress and restored neurotransmitter synthesis. Restoration of glutamate synthesis, which was independent of glial-neuronal recycling, depended on mitochondrial activity and led to an increase in gamma-amino butyric acid levels. These data indicate that parkin may be used as an alternative strategy to reduce Abeta levels and enhance autophagic clearance of Abeta-induced defects in AD. Parkin-mediated clearance of ubiquitinated Abeta may act in parallel with autophagy to clear molecular debris and defective mitochondria and restore neurotransmitter balance. |
