| First Author | Wang S | Year | 2019 |
| Journal | Neurophotonics | Volume | 6 |
| Issue | 4 | Pages | 045008 |
| PubMed ID | 31737743 | Mgi Jnum | J:352853 |
| Mgi Id | MGI:7706133 | Doi | 10.1117/1.NPh.6.4.045008 |
| Citation | Wang S, et al. (2019) Label-free multiphoton imaging of beta-amyloid plaques in Alzheimer's disease mouse models. Neurophotonics 6(4):045008 |
| abstractText | beta -Amyloid ( Abeta ) plaque, representing the progressive accumulation of the protein that mainly consists of Abeta , is one of the prominent pathological hallmarks of Alzheimer's disease (AD). Label-free imaging of Abeta plaques holds the potential to be a histological examination tool for diagnosing AD. We applied label-free multiphoton microscopy to identify extracellular Abeta plaque as well as intracellular Abeta accumulation for the first time from AD mouse models. We showed that a two-photon-excited fluorescence signal is a sensitive optical marker for revealing the spatial-temporal progression and the surrounding morphological changes of Abeta deposition, which demonstrated that both extracellular and intracellular Abeta accumulations play an important role in the progression of AD. Moreover, combined with a custom-developed image-processing program, we established a rapid method to visualize different degrees of Abeta deposition by color coding. These results provide an approach for investigating pathophysiology of AD that can complement traditional biomedical procedures. |
