Other
13 Authors
- Huang Q,
- Xiao J,
- Rominger A,
- Nitsch RM,
- Xie F,
- Zuo C,
- Maschio CA,
- Ni R,
- Guan Y,
- Shi K,
- Shi X,
- Konietzko U,
- Kong Y
| First Author | Kong Y | Year | 2024 |
| Journal | Mol Neurobiol | PubMed ID | 38502413 |
| Mgi Jnum | J:350687 | Mgi Id | MGI:7664334 |
| Doi | 10.1007/s12035-024-04106-7 | Citation | Kong Y, et al. (2024) Relationship Between Reactive Astrocytes, by [(18)F]SMBT-1 Imaging, with Amyloid-Beta, Tau, Glucose Metabolism, and TSPO in Mouse Models of Alzheimer's Disease. Mol Neurobiol |
| abstractText | Reactive astrocytes play an important role in the development of Alzheimer's disease (AD). Here, we aimed to investigate the temporospatial relationships among monoamine oxidase-B, tau and amyloid-beta (Abeta), translocator protein, and glucose metabolism by using multitracer imaging in AD transgenic mouse models. Positron emission tomography (PET) imaging with [(18)F]SMBT-1 (monoamine oxidase-B), [(18)F]florbetapir (Abeta), [(18)F]PM-PBB3 (tau), [(18)F]fluorodeoxyglucose (FDG), and [(18)F]DPA-714 (translocator protein) was carried out in 5- and 10-month-old APP/PS1, 11-month-old 3xTg mice, and aged-matched wild-type mice. The brain regional referenced standard uptake value (SUVR) was computed with the cerebellum as the reference region. Immunofluorescence staining was performed on mouse brain tissue slices. [(18)F]SMBT-1 and [(18)F]florbetapir SUVRs were greater in the cortex and hippocampus of 10-month-old APP/PS1 mice than in those of 5-month-old APP/PS1 mice and wild-type mice. No significant difference in the regional [(18)F]FDG or [(18)F]DPA-714 SUVRs was observed in the brains of 5- or 10-month-old APP/PS1 mice or wild-type mice. No significant difference in the SUVRs of any tracer was observed between 11-month-old 3xTg mice and age-matched wild-type mice. A positive correlation between the SUVRs of [(18)F]florbetapir and [(18)F]DPA-714 in the cortex and hippocampus was observed among the transgenic mice. Immunostaining validated the distribution of MAO-B and limited Abeta and tau pathology in 11-month-old 3xTg mice; and Abeta deposits in brain tissue from 10-month-old APP/PS1 mice. In summary, these findings provide in vivo evidence that an increase in astrocyte [(18)F]SMBT-1 accompanies Abeta accumulation in APP/PS1 models of AD amyloidosis. |
