| First Author | Dinkelmann M | Year | 2009 |
| Journal | Nat Struct Mol Biol | Volume | 16 |
| Issue | 8 | Pages | 808-13 |
| PubMed ID | 19633670 | Mgi Jnum | J:272543 |
| Mgi Id | MGI:6200200 | Doi | 10.1038/nsmb.1639 |
| Citation | Dinkelmann M, et al. (2009) Multiple functions of MRN in end-joining pathways during isotype class switching. Nat Struct Mol Biol 16(8):808-13 |
| abstractText | The Mre11-Rad50-NBS1 (MRN) complex has many roles in response to DNA double-strand breaks, but its functions in repair by nonhomologous end joining (NHEJ) pathways are poorly understood. We have investigated requirements for MRN in class switch recombination (CSR), a programmed DNA rearrangement in B lymphocytes that requires NHEJ. To this end, we have engineered mice that lack the entire MRN complex in B lymphocytes or that possess an intact complex that harbors mutant Mre11 lacking DNA nuclease activities. MRN deficiency confers a strong defect in CSR, affecting both the classic and the alternative NHEJ pathways. In contrast, absence of Mre11 nuclease activities causes a milder phenotype, revealing a separation of function within the complex. We propose a model in which MRN stabilizes distant breaks and processes DNA termini to facilitate repair by both the classical and alternative NHEJ pathways. |
