| First Author | Radice E | Year | 2020 |
| Journal | Cell Rep | Volume | 32 |
| Issue | 5 | Pages | 107951 |
| PubMed ID | 32755592 | Mgi Jnum | J:299944 |
| Mgi Id | MGI:6488886 | Doi | 10.1016/j.celrep.2020.107951 |
| Citation | Radice E, et al. (2020) Marginal Zone Formation Requires ACKR3 Expression on B Cells. Cell Rep 32(5):107951 |
| abstractText | The marginal zone (MZ) contributes to the highly organized spleen microarchitecture. We show that expression of atypical chemokine receptor 3 (ACKR3) defines two equal-sized populations of mouse MZ B cells (MZBs). ACKR3 is required for development of a functional MZ and for positioning of MZBs. Deletion of ACKR3 on B cells distorts the MZ, and MZBs fail to deliver antigens to follicles, reducing humoral responses. Reconstitution of MZ-deficient CD19(ko) mice shows that ACKR3(-) MZBs can differentiate into ACKR3(+) MZBs, but not vice versa. The lack of a MZ is rescued by adoptive transfer of ACKR3-sufficient, and less by ACKR3-deficient, follicular B cells (FoBs); hence, ACKR3 expression is crucial for establishment of the MZ. The inability of CD19(ko) mice to respond to T-independent antigen is rescued when ACKR3-proficient, but not ACKR3-deficient, FoBs are transferred. Accordingly, ACKR3-deficient FoBs are able to reconstitute the MZ if the niche is pre-established by ACKR3-proficient MZBs. |
