| First Author | de Alboran IM | Year | 2001 |
| Journal | Immunity | Volume | 14 |
| Issue | 1 | Pages | 45-55 |
| PubMed ID | 11163229 | Mgi Jnum | J:67222 |
| Mgi Id | MGI:1930073 | Doi | 10.1016/s1074-7613(01)00088-7 |
| Citation | de Alboran IM, et al. (2001) Analysis of C-MYC function in normal cells via conditional gene-targeted mutation. Immunity 14(1):45-55 |
| abstractText | Germline inactivation of c-myc in mice causes embryonic lethality. Therefore, we developed a LoxP/Cre-based conditional mutation approach to test the role of c-myc in mouse embryonic fibroblasts (MEFs) and mature B lymphocytes. Cre expression resulted in reduced proliferation of wild-type MEFs, but c-Myc-deficient MEFs showed a further reduction. In contrast to fibroblasts, Cre expression had no apparent affect on wild-type B cell proliferation. Deletion of both c-Myc genes in B cells led to severely impaired proliferation in response to anti-CD40 plus IL-4. However, treated cells did upregulate several early activation markers but not CD95 or CD95 ligand. We discuss these findings with respect to potential c-Myc functions in proliferation and apoptosis and also discuss potential limitations in the Cre-mediated gene inactivation approach. |
