| First Author | Chen S | Year | 2016 |
| Journal | Mol Cell Biol | Volume | 36 |
| Issue | 20 | Pages | 2543-52 |
| PubMed ID | 27457619 | Mgi Jnum | J:236307 |
| Mgi Id | MGI:5805719 | Doi | 10.1128/MCB.00150-16 |
| Citation | Chen S, et al. (2016) Id3 Orchestrates Germinal Center B Cell Development. Mol Cell Biol 36(20):2543-52 |
| abstractText | Previous studies have demonstrated that E proteins induce activation-induced deaminase (AID) expression in activated B cells. Here, we examined the role of Id3 in germinal center (GC) cells. We found that Id3 expression is high in follicular B lineage cells but declines in GC cells. Immunized mice with Id3 expression depleted displayed a block in germinal center B cell maturation, showed reduced numbers of marginal zone B cells and class-switched cells, and were associated with decreased antibody titers and lower numbers of plasma cells. In vitro, Id3-depleted B cells displayed a defect in class switch recombination. Whereas AID levels were not altered in Id3-depleted activated B cells, the expression of a subset of genes encoding signaling components of antigen receptor-, cytokine receptor-, and chemokine receptor-mediated signaling was significantly impaired. We propose that during the GC reaction, Id3 levels decline to activate the expression of genes encoding signaling components that mediate B cell receptor- and or cytokine receptor-mediated signaling to promote the differentiation of GC B cells. |
