| First Author | Jin HY | Year | 2017 |
| Journal | PLoS Genet | Volume | 13 |
| Issue | 2 | Pages | e1006623 |
| PubMed ID | 28241004 | Mgi Jnum | J:242075 |
| Mgi Id | MGI:5904388 | Doi | 10.1371/journal.pgen.1006623 |
| Citation | Jin HY, et al. (2017) Differential Sensitivity of Target Genes to Translational Repression by miR-17~92. PLoS Genet 13(2):e1006623 |
| abstractText | MicroRNAs (miRNAs) are thought to exert their functions by modulating the expression of hundreds of target genes and each to a small degree, but it remains unclear how small changes in hundreds of target genes are translated into the specific function of a miRNA. Here, we conducted an integrated analysis of transcriptome and translatome of primary B cells from mutant mice expressing miR-17~92 at three different levels to address this issue. We found that target genes exhibit differential sensitivity to miRNA suppression and that only a small fraction of target genes are actually suppressed by a given concentration of miRNA under physiological conditions. Transgenic expression and deletion of the same miRNA gene regulate largely distinct sets of target genes. miR-17~92 controls target gene expression mainly through translational repression and 5'UTR plays an important role in regulating target gene sensitivity to miRNA suppression. These findings provide molecular insights into a model in which miRNAs exert their specific functions through a small number of key target genes. |
