| First Author | Audzevich T | Year | 2017 |
| Journal | Proc Natl Acad Sci U S A | Volume | 114 |
| Issue | 20 | Pages | E3954-E3963 |
| PubMed ID | 28461481 | Mgi Jnum | J:242211 |
| Mgi Id | MGI:5904686 | Doi | 10.1073/pnas.1616417114 |
| Citation | Audzevich T, et al. (2017) Pre/pro-B cells generate macrophage populations during homeostasis and inflammation. Proc Natl Acad Sci U S A 114(20):E3954-E3963 |
| abstractText | Most tissue-resident macrophages (Mphis) are believed to be derived prenatally and are assumed to maintain themselves throughout life by self-proliferation. However, in adult mice we identified a progenitor within bone marrow, early pro-B cell/fraction B, that differentiates into tissue Mphis. These Mphi precursors have non-rearranged B-cell receptor genes and coexpress myeloid (GR1, CD11b, and CD16/32) and lymphoid (B220 and CD19) lineage markers. During steady state, these precursors exit bone marrow, losing Gr1, and enter the systemic circulation, seeding the gastrointestinal system as well as pleural and peritoneal cavities but not the brain. While in these tissues, they acquire a transcriptome identical to embryonically derived tissue-resident Mphis. Similarly, these Mphi precursors also enter sites of inflammation, gaining CD115, F4/80, and CD16/32, and become indistinguishable from blood monocyte-derived Mphis. Thus, we have identified a population of cells within the bone marrow early pro-B cell compartment that possess functional plasticity to differentiate into either tissue-resident or inflammatory Mphis, depending on microenvironmental signals. We propose that these precursors represent an additional source of Mphi populations in adult mice during steady state and inflammation. |
