| First Author | Hobeika E | Year | 2006 |
| Journal | Proc Natl Acad Sci U S A | Volume | 103 |
| Issue | 37 | Pages | 13789-94 |
| PubMed ID | 16940357 | Mgi Jnum | J:113645 |
| Mgi Id | MGI:3687374 | Doi | 10.1073/pnas.0605944103 |
| Citation | Hobeika E, et al. (2006) Testing gene function early in the B cell lineage in mb1-cre mice. Proc Natl Acad Sci U S A 103(37):13789-94 |
| abstractText | The mb1 gene encodes the Ig-alpha signaling subunit of the B cell antigen receptor and is expressed exclusively in B cells beginning at the very early pro-B cell stage in the bone marrow. We examine here the efficacy of the mb1 gene as a host locus for cre recombinase expression in B cells. We show that by integrating a humanized cre recombinase into the mb1 locus we obtain extraordinarily efficient recombination of loxP sites in the B cell lineage. The results from a variety of reporter genes including the splicing factor SRp20 and the DNA methylase Dnmt1 suggest that mb1-cre is probably the best model so far described for pan-B cell-specific cre expression. The availability of a mouse line with efficient cre-mediated recombination at an early developmental stage in the B lineage provides an opportunity to study the role of various genes specifically in B cell development and function. |
