| First Author | Cheuk E | Year | 2005 |
| Journal | Cell Immunol | Volume | 234 |
| Issue | 2 | Pages | 110-23 |
| PubMed ID | 16054612 | Mgi Jnum | J:100542 |
| Mgi Id | MGI:3588819 | Doi | 10.1016/j.cellimm.2005.06.004 |
| Citation | Cheuk E, et al. (2005) Strong memory CD8(+) T cell responses against immunodominant and three new subdominant HLA-B27-restricted influenza A CTL epitopes following secondary infection of HLA-B27 transgenic mice. Cell Immunol 234(2):110-23 |
| abstractText | We previously showed that the known HLA-B27-restricted influenza A epitope identified from human studies, NP.383-391, was recognized by CTLs following influenza A infection of transgenic (Tg) HLA-B27/H2 class I-deficient (H2 DKO) mice. Here, we examined the kinetics of the primary NP.383-391-specific response in Tg HLA-B27/H2 DKO mice at the site of respiratory infection, along with the profile of additional influenza A epitopes recognized. While the temporal kinetics of the Tg HLA-B27/NP.383-391-specific CD8(+) T cell response paralleled the H2-D(b)/NP.366-374-specific response of non-Tg H2(b) mice, the magnitude was less. Using epitope prediction programs, we identified three novel B27-restricted influenza A epitopes, PB2.702-710, PB1.571-579, and PB2.368-376, recognized during both the primary and secondary response to infection. Although the secondary NP.383-391-specific response was dominant, PB1.571-579 and PB2.368-376 stimulated stronger proliferative expansion in memory T cells. Our results indicate a broader B27/influenza A CTL repertoire than previously known. Together with results for other HLA class I alleles, this information will become important in improving vaccine strategies for influenza A and other human pathogens. |
