| First Author | Lesnikova A | Year | 2021 |
| Journal | J Neurosci | Volume | 41 |
| Issue | 5 | Pages | 972-980 |
| PubMed ID | 33293360 | Mgi Jnum | J:302570 |
| Mgi Id | MGI:6506129 | Doi | 10.1523/JNEUROSCI.2228-20.2020 |
| Citation | Lesnikova A, et al. (2021) Chondroitinase and Antidepressants Promote Plasticity by Releasing TRKB from Dephosphorylating Control of PTPsigma in Parvalbumin Neurons. J Neurosci 41(5):972-980 |
| abstractText | Perineuronal nets (PNNs) are an extracellular matrix structure rich in chondroitin sulfate proteoglycans (CSPGs), which preferentially encase parvalbumin-containing (PV(+)) interneurons. PNNs restrict cortical network plasticity but the molecular mechanisms involved are unclear. We found that reactivation of ocular dominance plasticity in the adult visual cortex induced by chondroitinase ABC (chABC)-mediated PNN removal requires intact signaling by the neurotrophin receptor TRKB in PV(+) neurons. Additionally, we demonstrate that chABC increases TRKB phosphorylation (pTRKB), while PNN component aggrecan attenuates brain-derived neurotrophic factor (BDNF)-induced pTRKB in cortical neurons in culture. We further found that protein tyrosine phosphatase sigma (PTPsigma, PTPRS), receptor for CSPGs, interacts with TRKB and restricts TRKB phosphorylation. PTPsigma deletion increases phosphorylation of TRKB in vitro and in vivo in male and female mice, and juvenile-like plasticity is retained in the visual cortex of adult PTPsigma-deficient mice (PTPsigma(+/-)). The antidepressant drug fluoxetine, which is known to promote TRKB phosphorylation and reopen critical period-like plasticity in the adult brain, disrupts the interaction between TRKB and PTPsigma by binding to the transmembrane domain of TRKB. We propose that both chABC and fluoxetine reopen critical period-like plasticity in the adult visual cortex by promoting TRKB signaling in PV(+) neurons through inhibition of TRKB dephosphorylation by the PTPsigma-CSPG complex.SIGNIFICANCE STATEMENT Critical period-like plasticity can be reactivated in the adult visual cortex through disruption of perineuronal nets (PNNs) by chondroitinase treatment, or by chronic antidepressant treatment. We now show that the effects of both chondroitinase and fluoxetine are mediated by the neurotrophin receptor TRKB in parvalbumin-containing (PV(+)) interneurons. We found that chondroitinase-induced visual cortical plasticity is dependent on TRKB in PV(+) neurons. Protein tyrosine phosphatase sigma (PTPsigma, PTPRS), a receptor for PNNs, interacts with TRKB and inhibits its phosphorylation, and chondroitinase treatment or deletion of PTPsigma increases TRKB phosphorylation. Antidepressant fluoxetine disrupts the interaction between TRKB and PTPsigma, thereby increasing TRKB phosphorylation. Thus, juvenile-like plasticity induced by both chondroitinase and antidepressant treatment is mediated by TRKB activation in PV(+) interneurons. |
