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Publication : Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers.

First Author  Amberg N Year  2021
Journal  STAR Protoc Volume  2
Issue  4 Pages  100939
PubMed ID  34825212 Mgi Jnum  J:350011
Mgi Id  MGI:7660909 Doi  10.1016/j.xpro.2021.100939
Citation  Amberg N, et al. (2021) Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers. STAR Protoc 2(4):100939
abstractText  Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis. For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).
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