| First Author | Haering C | Year | 2015 |
| Journal | J Biol Chem | Volume | 290 |
| Issue | 15 | Pages | 9767-79 |
| PubMed ID | 25713142 | Mgi Jnum | J:243530 |
| Mgi Id | MGI:5908782 | Doi | 10.1074/jbc.M115.640656 |
| Citation | Haering C, et al. (2015) Ion transporter NKCC1, modulator of neurogenesis in murine olfactory neurons. J Biol Chem 290(15):9767-79 |
| abstractText | Olfaction is one of the most crucial senses for vertebrates regarding foraging and social behavior. Therefore, it is of particular interest to investigate the sense of smell, its function on a molecular level, the signaling proteins involved in the process and the mechanism of required ion transport. In recent years, the precise role of the ion transporter NKCC1 in olfactory sensory neuron (OSN) chloride accumulation has been a controversial subject. NKCC1 is expressed in OSNs and is involved in chloride accumulation of dissociated neurons, but it had not been shown to play a role in mouse odorant sensation. Here, we present electro-olfactogram recordings (EOG) demonstrating that NKCC1-deficient mice exhibit significant defects in perception of a complex odorant mixture (Henkel100) in both air-phase and submerged approaches. Using next generation sequencing (NGS) and RT-PCR experiments of NKCC1-deficient and wild type mouse transcriptomes, we confirmed the absence of a highly expressed ion transporter that could compensate for NKCC1. Additional histological investigations demonstrated a reduced number of cells in the olfactory epithelium (OE), resulting in a thinner neuronal layer. Therefore, we conclude that NKCC1 is an important transporter involved in chloride ion accumulation in the olfactory epithelium, but it is also involved in OSN neurogenesis. |
