| First Author | Ruiz-Herguido C | Year | 2012 |
| Journal | J Exp Med | Volume | 209 |
| Issue | 8 | Pages | 1457-68 |
| PubMed ID | 22802352 | Mgi Jnum | J:189158 |
| Mgi Id | MGI:5444556 | Doi | 10.1084/jem.20120225 |
| Citation | Ruiz-Herguido C, et al. (2012) Hematopoietic stem cell development requires transient Wnt/beta-catenin activity. J Exp Med 209(8):1457-68 |
| abstractText | Understanding how hematopoietic stem cells (HSCs) are generated and the signals that control this process is a crucial issue for regenerative medicine applications that require in vitro production of HSC. HSCs emerge during embryonic life from an endothelial-like cell population that resides in the aorta-gonad-mesonephros (AGM) region. We show here that beta-catenin is nuclear and active in few endothelial nonhematopoietic cells closely associated with the emerging hematopoietic clusters of the embryonic aorta during mouse development. Importantly, Wnt/beta-catenin activity is transiently required in the AGM to generate long-term HSCs and to produce hematopoietic cells in vitro from AGM endothelial precursors. Genetic deletion of beta-catenin from the embryonic endothelium stage (using VE-cadherin-Cre recombinase), but not from embryonic hematopoietic cells (using Vav1-Cre), precludes progression of mutant cells toward the hematopoietic lineage; however, these mutant cells still contribute to the adult endothelium. Together, those findings indicate that Wnt/beta-catenin activity is needed for the emergence but not the maintenance of HSCs in mouse embryos. |
